INTRODUCTION:

Modern lifestyles depend heavily on cosmetics, and the use of natural products is becoming more and more popular. Herbal cosmetics, which have been used for centuries in traditional medicine, are becoming more and more well-liked due to their antibacterial and anti-inflammatory properties. Compared to synthetic alternatives, herbal products are thought to be safer. The large range of lip balms and lipsticks that are currently on the market reflects the evolution of lip colouring, an age-old technique, with a greater choice of tints, textures, and finishes¹.

Since the lips are the only area of the body without pores, they are a vital part of the face and need to be well nourished and hydrated. Lip balms, serums, rouges, oils, masks, scrubs, lipsticks, and exfoliators are examples of cosmetics that have been created to shield the lips from irritation, dehydration, and hyperpigmentation. In addition to improving appearance, these products aid in the healing of skin disorders such inflammation, cracking, chapping, and dryness².

Lip balm is a waxy product that is intended to protect and moisturize the lips from environmental irritants, dryness, and cold temperatures. It forms a protective coating on the lips, preventing infection, inflammation, and chapping. In addition to reducing irritation, occlusive lip balms stop saliva from moisturizing the lips. Lip balm components keep lips hydrated and help chapped lips heal. Lip balm is generally less expensive than lipstick and is gender-neutral. It is frequently used to assist set matte lipstick, providing a quick and affordable method of hydrating and shielding the lips from the elements³.

In the Ayurvedic formula Shata Dhauta Ghrita, "Shata" means "one hundred," "Dhauta" means "washing," and "Ghrita" means "ghee." This method turns ghee into a cooling, smooth, and non-greasy cream by washing it in water up to 100 times. This method makes ordinary ghee easier to apply by eliminating its potent fragrance and gritty texture. Unlike ghee, which has an acidic pH, Shata Dhauta Ghrita has a neutral pH, preventing skin irritation. Particle size is decreased by washing, resulting in a consistent, non-sticky product that improves skin absorption. The procedure improves viscosity and consistency by creating a smooth oil-in-water emulsion. In comparison with regular ghee, Shata Dhauta Ghrita offers a softer alternative^4-6.

MATERIALS AND METHODS:

Materials: A2 cow ghee, Moringa oil, Mulberry oil, Aloe vera gel, Bees Wax, Coco Butter, Lychee Oil, Sudan Red.

Table No. 1: Raw Material with Their Uses

Sr. No.	Raw Material	Uses
1.	A2 cow ghee	Moisturizing, Nourishing
2.	Moringa oil	Antioxidant, Antiaging
3.	Mulberry oil	Even Tone:
4.	Aloe vera gel	Soothing effect, Reduce Hyperpigmentation
5.	Bees Wax	Thickening Agent
6.	Coco Butter	Healing and Repair
7.	Sudan Red	Colouring Agent
8.	Lychee Oil	Flavouring Agent

Preparation of Base Using A2 Cow Ghee⁶.

Perform these procedures to make Shata Dhauta Ghrita the Niragni method (without heat):

Components:

Make by using fresh ghee.

Make sure the water you use for washing is gentle and pure.

Step 1: Mix water and ghee: Combine the ghee and water in a clean container in equal parts (1:1 ratio)

Step 2: Mix the ingredients: In a copper vessel, continuously stir the ghee and water, and wash the ghee 100 times with consistent stirring.

Step 3: Allow the separation: Allow the ghee to naturally separate from the water by allowing the mixture settle for a few minutes.

Step 4: Drain the water: Hold the ghee in the container and carefully drain off the water.

Step 5: Carry out the procedure again: To smooth out and purify the ghee, repeat the washing and separation procedure 10 times in total.

Step 6: Completion of Shata Dhauta Ghrita: Following 10 cycles, the ghee is completely cleansed and prepared for use in Ayurvedic medicines as Shata dhauta Ghrita.

This technique ensures the ghee's purity and medicinal qualities by repeatedly cleaning it without using heat.

Phytoconstituent Analysis:

Phytoconstituent analysis revealed that Moringa oil contains flavonoids, tannins, and vitamins C and E, offering strong anti-aging and antioxidant benefits. Mulberry oil contains tannins, alkaloids, and vitamins C and E, which provide effective anti-acne properties. Aloe vera gel has lignins, amino acids, flavonoids, and anthraquinones, which soothe the skin and help reduce hyperpigmentation.

Preparation of Lip Balm:⁷

Step 1: Weigh Ingredients: Weigh Beeswax, Sudan red, Lychee oil, Moringa oil, Mulberry oil, Aloe vera gel, and A2 cow ghee precisely.

Step 2: Melt Ghee and Beeswax: Using indirect heat, melt A2 cow ghee and beeswax in a beaker, being careful not to let the temperature rise above 90°C.

Step 3: Add Oils: Gently whisk in Aloe vera gel, Mulberry oil, and Moringa oil until smooth and uniform.

Step 4: Add Colour Agent: Mix the colouring agent, Sudan red, until it is well combined.

Step 5: Add Flavour Agent: Add the flavouring agent, Lychee oil, and mix constantly to ensure even distribution.

Step 6: Pour and Cool: To solidify, pour the liquid into lip balm containers and set them in an ice bath in a cool, dry location away from the sun for approximately an hour.

Formulation Table:

Table No. 2: Formulation Table with Their Prescribed Quantity

Sr. No.	Ingredients	F1	F2	F3
1.	Ghee	4g	10g	20g
2.	Moringa oil	1.5g	3.75g	7.50g
3.	Mulberry oil	1.5g	3.75g	7.50g
4.	Aloe vera gel	4g	10g	20g
5.	Bees Wax	1g	2.5g	5g
6.	Coco Butter	0.2g	0.5g	1g
7.	Sudan red	0.8g	2g	4g
8.	Lychee oil	1.5ml	15ml	30ml

EVALUATION TEST:

1. Organoleptic Evaluation:⁸

Colour: Uniform, attractive, and without any defects. Odour: A delicate, pleasant aroma free from any unpleasant smells.

Texture: Smooth and soft, free from any graininess or roughness.

Consistency: Precisely the proper amount of thickness, easy to apply, and neither too soft nor too stiff.

2. pH Test:^8,9

Using a buffer solution, the pH meter was calibrated. The pH was measured by dissolving 1gram of the substance in 100ml of water. The pH was then determined using a pH meter.

3. Spreadability Test:^8,9

After placing two glass slides with enough lip balm, the top slide is subjected to a 100-gram weight for five minutes. The movement of the upper slide over the lower slide or the amount of time it takes for the slides to separate are two ways to measure spreadability. The following formula is used to determine the spreadability:

S = m × l / t

Where,

m is the weight on the upper slide,

l is the distance the upper slide has gone,

t is the time required.

4. Melting Point Test:^8,9

The drug-containing capillary was submerged in liquid paraffin inside the melting point equipment, which had a magnetic stirring mechanism. Observations of the melting point were made and noted accurately.

5. Irritancy Test:^8,9

A small amount of the prepared product is applied to the dorsal surface of the skin of the left hand for 10 minutes in order to conduct this test. The skin is then examined for any indications of rash, edema, erythema, or inflammation.

6. Surface Anomalies:⁹

This was examined for surface defects in the lip balm formulation to make sure there were no crystal formations or mold, fungal, or other impurity contaminations.

7. Aging Stability:⁹

The product was stored at 40°C for an hour in order to evaluate its stability. After that, a number different variables were examined, including bleeding, surface crystallization, and application efficiency.

8. Stability Test:⁸

The prepared lip balm was tested over a 30-day period at different temperatures, including room temperature (25°C), refrigeration (5°C), and increased temperature (40°C), in order to speed up the research. Its spreadability, pH, and organoleptic properties were evaluated during this time.

Result and Discussion:

1. Organoleptic Evaluation:

Evaluations were conducted on the lip balm's colour, odour, texture, and consistency.

Table No. 3: Organoleptic Evaluation

Test	F1	F2	F3
Colour	Reddish orange	Reddish orange	Reddish orange
Odour	Fruity	Fruity	Fruity
Texture	Smooth	Smooth	Smooth
Consistency	Solid	Solid	Solid

2. Phytoconstituent Evaluation:

Table No. 4: Phytoconstituent Evaluation

Name	Phytoconstituent	Test	Result
Moringa oil	Flavonoids	Alkaline Reagent Test
	Tannins	Ferric Chloride Test
	Vitamin C	Ferric Chloride Test
	Vitamin E	Iodine Test
Mulberry oil	Tannins	Ferric Chloride Test
	Alkaloids	Dragendorff’s Test
	Vitamin C	Ferric Chloride Test
	Vitamin E	Iodine Test
Aloe vera gel	Lignins	Phenolic Test
	Amino Acids	Ninhydrin Test
	Flavonoids	Alkaline Reagent Test
	Anthraquinones	Borntrager’s Test

3. Physical Evaluation:

The lip balm formulas (F1, F2, and F3) revealed a pH that was within the standard range of 4.5 to 7.5. Melting points vary between 50°C and 60°C. They did not have superficial defects, was non-irritating, and had high spreadability. It appears that all formulations stayed stable during stability testing and kept their smooth texture over time indicates they are safe, stable, and effective for usage.

Table No. 5: Physical Evaluation

Test	F1	F2	F3
pH
Spreadability	Good	Good	Good
Melting point
Irritancy	No	No	No
Surface Anomalies	No	No	No
Aging Stability	Smooth	Smooth	Smooth
Stability Test	Stable	Stable	Stable

CONCLUSION:

This study evaluates the effectiveness and the production of a herbal lip balm using ingredients like aloe vera gel, mulberry oil, moringa oil, and A2 cow ghee. Shata Dhauta Ghrita, an Ayurvedic technique, was used to make the lip balm. According to tests, the lip balm's color, texture, odor, and consistency were all good. It was stable, non-irritating, had a melting point of 50–60°C, good spreadability, and a pH in the normal range. There were no surface irregularities or irritations recognized, and the formulations were stable, safe, and effective for use.

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Received on 03.05.2025 Revised on 23.08.2025

Accepted on 27.10.2025 Published on 31.01.2026

Available online from February 07, 2026

Res. J. Pharmacognosy and Phytochem. 2026; 18(1):1-4.

DOI: 10.52711/0975-4385.2026.00001

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